名稱 | TLR9/NFkB-Luc/HEK293 |
型號 | CBP74091 |
報價 | |
特點 | TLR9/NFkB-Luc/HEK293,凍存條件:90% FBS+10% DMSO;培養(yǎng)基:MEM+10%FBS+1% non-essential amino acids(NEAA)+1 mM Na pyruvate+50 μg/ml Hygromycin B+2ug/ml puromycin |
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- 詳細內容
I. Background | |
Toll 樣受體(TLR)是一種模式識別受體(PRR),高度保守,可識別各種類型的微 生物病原體相關模式分子(PAMP)。在人類中發(fā)現(xiàn)了 10 個 Toll 樣受體家族(TLR1-10) 成員,在小鼠中發(fā)現(xiàn)了 12 個成員(TLR1-9、TLR11 and TLR13)。TLR1、TLR2、TLR4、 TLR5 和 TLR6 分布在細胞膜表面;TLR3、TLR7、TLR8、TLR9 和 TLR10 分布在細胞內 涵體膜表面(TLR3 識別 dsRNA,TLR9 識別 dsDNA)。TLRs 下游信號的激活主要依賴于 兩類轉錄因子:NF-κB 和干擾調節(jié)因子(IRFs),它們主要誘導促炎細胞因子和 I 型干擾 素(IFN)的產生。 | |
II. Description | |
TLR9 NFκB-Luc HEK293 報告基因藥靶模型很好的模擬了體內 TLR9 的信號轉導過程, 原理見下圖所示。
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II. Introduction | |
Host Cell: | HEK293 |
Expressed gene: | TLR9、NFκB-Luc |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | TLR, TLR-9, tlr, nfkb, nf-kb |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | MEM+10%FBS+1% NEAA+1 mM NaP+50 μg/ml hygromycin+2 μg/ml puromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
III. Description of Host Cell Line | |
Organism: | Human |
Tissue: | Embryonic kidney |
Disease: | Normal |
Morphology: | Epitheloid cell |
Growth Properties: | Adherent |
IV. Representative Data |
Figure 2.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Dose Response of ODN 2006 in TLR9 NFκB-Luc HEK293(C3).